期刊
SCIENCE AND TECHNOLOGY OF ARCHAEOLOGICAL RESEARCH
卷 3, 期 1, 页码 58-70出版社
ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD
DOI: 10.1080/20548923.2017.1361629
关键词
Archaeology; dental calculus; destructive analysis; LC-MS/MS; shotgun proteomics; protein preservation
类别
资金
- Wellcome Trust [108375/Z/15/Z]
- University of York - Wellcome Trust [097829/Z/11/A]
- Max Planck Society
- Arts and Humanities Research Council [AH/N005015/1]
- AHRC [1802124] Funding Source: UKRI
Proteomic analysis of dental calculus is emerging as a powerful tool for disease and dietary characterisation of archaeological populations. To better understand the variability in protein results from dental calculus, we analysed 21 samples from three Roman-period populations to compare: 1) the quantity of extracted protein; 2) the number of mass spectral queries; and 3) the number of peptide spectral matches and protein identifications. We found little correlation between the quantity of calculus analysed and total protein identifications, as well as no systematic trends between site location and protein preservation. We identified a wide range of individual variability, which may be associated with the mechanisms of calculus formation and/or post-depositional contamination, in addition to taphonomic factors. Our results suggest dental calculus is indeed a stable, long-term reservoir of proteins as previously reported, but further systematic studies are needed to identify mechanisms associated with protein entrapment and survival in dental calculus.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据