期刊
BIOTECHNOLOGY PROGRESS
卷 30, 期 5, 页码 1185-1189出版社
WILEY-BLACKWELL
DOI: 10.1002/btpr.1940
关键词
bioreactor; catalytic antibody 14D9; cell suspension cultures; hairy roots; in vitro plant cell cultures; Nicotiana tabacum; protein additives
资金
- Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT) from Argentina through the PICT [00552]
The effect of two protein stabilizers (polyvinylpyrrolidone [PVP] and gelatine) on growth and 14D9 yield of Nicotiana tabacum cell suspension cultures (Ab-KDEL and sec-Ab) was analyzed. The addition of PVP at a concentration of 1.0 g L-1 produced the highest total 14D9 yield (biomass + culture medium) in the Ab-KDEL line (4.82% total soluble protein [TSP]). With the addition of gelatine, the highest total 14D9 yield (2.48% TSP) was attained in the Ab-KDEL line at 5.0 g L-1 gelatine. When the Ab-KDEL suspended cells were cultured in a 2-L bioreactor, the highest 14D9 yield was 8.1% TSP at a 5% w/v inoculum size, which was the best 14D9 yield so far obtained in the platforms tested (E. coli, N. tabacum leaves and seeds, N. tabacum hairy roots, and cell suspension cultures). (C) 2014 American Institute of Chemical Engineers
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