期刊
BIOTECHNOLOGY PROGRESS
卷 29, 期 3, 页码 681-687出版社
WILEY-BLACKWELL
DOI: 10.1002/btpr.1725
关键词
Chlorella pyrenoidosa; polypeptides; antitumor; encapsulation; controlled release
资金
- Scientific and Technological Research and Development Program of Guangdong Province [2009B090300271, 2011B090400537]
- Scientific and Technological Research Program of Guangzhou City [2012J4100123]
Chlorella pyrenoidosa is a unicellular green algae and has been a popular foodstuff worldwide. However, no reports on the antitumor peptides from such a microalgae are available in the literature. In this study, using low-temperature high-pressure extraction, enzymatic hydrolysis, ion exchange, and gel filtration chromatography, we separated a polypeptide that exhibited inhibitory activity on human liver cancer HepG2 cells, and named the polypeptide CPAP (C. pyrenoidosa antitumor polypeptide). Furthermore, the micro- and nanoencapsulation of CPAP were investigated by using two methods: complex coacervation and ionotropic gelation. The in vitro release tests revealed that CPAP was well preserved against gastric enzymatic degradation after micro/nanoencapsulation and the slowly controlled release in the intestine could be potentially achieved. These results suggest that CPAP may be a useful ingredient in food, nutraceutical, and pharmaceutical applications. (c) 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:681-687, 2013
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