期刊
BIOTECHNOLOGY PROGRESS
卷 27, 期 2, 页码 530-538出版社
WILEY
DOI: 10.1002/btpr.544
关键词
flow injection analysis; glucose biomonitoring; glucose oxidase; pyranose oxidase; self-assembled monolayers
资金
- Scientific and Technological Research Council of Turkey (TUBITAK)
- Federal Ministry of Education and Research (BMBF) [108T293]
- EBILTEM (Ege University, Research and Application Center of Science and Technology)
- Ege University Research Foundation [2009 Fen 0059]
This article deals with the use of pyranose oxidase (PyOx) and glucose oxidase (GOx) enzymes in amperometric biosensor design and their application in monitoring fermentation processes with the combination of flow injection analysis (FIA). The amperometric studies were carried out at -0.7 V by following the oxygen consumption due to the enzymatic reactions for both batch and FIA modes. Optimization studies (enzyme amounts and pH) and analytical parameters such as linearity, repeatability, effect of interference, storage, and operational stabilities have been studied. Under optimized conditions, for the PyOx-based biosensor, linear graph was obtained from 0.025 to 0.5 mM glucose in phosphate buffer ( 50 mM) at pH 7.0 with the equation of y = 3.358x + 0.028 and R-2 = 0.998. Linearity was found to be 0.01-1.0 mM in citrate buffer (50 mM and pH 4.0) with the equation of y = 1.539x + 0.181 and R-2 = 0.992 for the GOx biosensor. Finally, these biosensor configurations were further evaluated in a conventional flow injection system. Results from batch experiments provide a guide to design sensitive, stable, and interference-free biosensors for FIA mode. Biosensor stability, dynamic range, and repeatability were also studied in FIA conditions, and the applicability for the determination of glucose in fermentation medium could be successfully demonstrated. The FIA-combined glucose biosensor was used for the offline monitoring of yeast fermentation. The obtained results correlated well with HPLC measurements. (C) 2011 American Institute of Chemical Engineers Biotechnol. Prog., 27: 530-538, 2011
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据