期刊
BIOTECHNOLOGY PROGRESS
卷 25, 期 1, 页码 124-131出版社
WILEY
DOI: 10.1002/btpr.100
关键词
N-acetyl-D-glucosamine (GlcNAc); Aeromonas schubertii; endochitinase; hexameric chitin oligosaccharide; SDS-resistance; in-gel chitinase assay
资金
- National Science Council, Taiwan [NSC91-2320-B13t-001, NSC-23-B-182-075]
- NATIONAL CENTER FOR RESEARCH RESOURCES [P41RR000954] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P41GM103422] Funding Source: NIH RePORTER
Two SDS-resistant endochitinases, designated as ASCH153 and ASCH161, were isolated from Aeromonas schubertii in a soil sample from southern Taiwan. MALDI-TOF mass measurement indicates the molecular weights of 53,527 for ASCH153 and 61,202 for ASCHI61. N-terminal and internal amino acid sequences were obtained, and BLAST analysis of the sequences and MS/MS peptide sequencing showed that they were novel proteins. Degradation of chitin by these two endochitinases gave rise to hexameric chitin oligosaccharide, a compound known to have several potent biomedical functions. ASCH153 and ASCH161 retained, respectively, 65% and 75%, of their chitinase activity in the presence of 5% SDS and 100% of their activity in the presence of 10% beta-mercaptoethanol. These results demonstrate that they are SDS-resistant endochitinases and probably have a rigid structure. 0 2009 American Institute of Chemical Engineers Biotechnol. Prog., 25: 124-431, 2009
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