期刊
BIOTECHNOLOGY LETTERS
卷 36, 期 10, 页码 2069-2077出版社
SPRINGER
DOI: 10.1007/s10529-014-1578-2
关键词
Corynebacterium Glutamicum; Metabolic Engineering; Monoterpene; Pinene; Toxicity
资金
- National Research Foundation of Korea - Korean Government (Ministry of Science, ICT & Future Planning)
- Creative Allied Program (CAP) of the Korea Research Council of Fundamental Science and Technology (KRCF)/Korea Institute of Science and Technology (KIST) [2E24832]
- National Research Council of Science & Technology (NST), Republic of Korea [2E24830] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [특화전문대학원-01] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Pinene is a monoterpenes (C-10) that is produced in a genetically-engineered microbial host for its industrial applications in fragrances, flavoring agents, pharmaceuticals, and biofuels. Herein, we have metabolically-engineered Corynebacterium glutamicum, to produce pinene and studied its toxicity in C. glutamicum. Geranyl diphosphate synthases (GPPS) and pinene synthases (PS), obtained from Pinus taeda and Abies grandis, were co-expressed with over-expressed native 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and isopentenyl diphosphate isomerase (Idi) from C. glutamicum using CoryneBrick vector. Most strains expressing PS-GPPSs produced detectable amounts of pinene, but co-expression of DXS and IDI with PS (P. taeda) and GPPS (A. grandis) resulted in 27 mu g +/- A 7 alpha-pinene g(-1) cell dry weight, which is the first report in C. glutamicum. Further engineering of PS and GPPS in the C. glutamicum strain may increase pinene production.
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