期刊
BIOTECHNOLOGY LETTERS
卷 37, 期 5, 页码 1021-1030出版社
SPRINGER
DOI: 10.1007/s10529-014-1758-0
关键词
Bioinformatics; Biomass; Chlorella vulgaris; Heterotrophic-Na+ induction; Lipid; Proteomics
资金
- National Natural Science Foundation of China [21306161]
- China Postdoctoral Science Foundation [2012M521531, 2014T70775]
- Hunan Provincial Natural Science Foundation of China [13JJ3062]
A heterotrophic-Na+ induction (HNI) two-step regime was developed to enhance lipid accumulation in oleaginous Chlorella vulgaris. C. vulgaris was cultivated heterotrophically to a biomass of 7.8 g l(-1) in 120 h. The cells were re-suspended in fresh media supplemented with 0.5 M NaCl followed by 12 h growth to accumulate lipid to 53.4 % (w/w). The lipid productivity (625 mg l(-1) day(-1)) achieved with HNI was better than that using heterotrophy alone (405 mg l(-1) day(-1)). To promote possible applications of HNI strategy in other microalgal species, the lipid triggers and potential molecular pathways associated with lipid biosynthesis were investigated. Malic enzyme and acyl-CoA-binding protein were key metabolic checkpoints found to modulate lipid biosynthesis in cells. These results provide the foundation to develop high-lipid engineering miroalgae for industrialization of biodiesel.
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