期刊
BIOTECHNOLOGY LETTERS
卷 36, 期 8, 页码 1615-1622出版社
SPRINGER
DOI: 10.1007/s10529-014-1521-6
关键词
Arg-Val-Phe; Apoptosis; Human neuroblastoma cells; Oxidative stress; Peptide; Poly(ADP-ribose) polymerase; Wheat germ peptide (RVF)
资金
- National Natural Science Foundation of China [31171672, 31071523]
- Key Programs from MOST [2012BAD34B02, 2011BAK10B05, 2012BAD29B05]
- Public Program from the General Administration of Quality Supervision [201310128]
- Ministry of Health Foundation of China [W201304]
RVF (Arg-Val-Phe), a peptide derived from wheat germ, shows antioxidant properties. Here, the neuroprotective efficacies of RVF were investigated in human neuroblastoma cells (SH-SY5Y) that were pretreated with RVF (150-250 mu M, 4 h) and exposed to H2O2 (200 mu M). RVF increased viable cell numbers by 37 % and reduced the release of lactate dehydrogenase. Pretreatment with RVF also inhibited H2O2-induced accumulation of reactive oxygen species and maintained the mitochondrial transmembrane potential as well as preventing intracellular Ca2+ dysregulation during H2O2 exposure. Furthermore, pretreatment with RVF increased the Bcl-2/Bax ratio and blocked cleavage poly(ADP-ribose) polymerase by inhibiting caspase-3 activation, thus decreasing apoptosis.
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