期刊
BIOTECHNOLOGY LETTERS
卷 36, 期 4, 页码 775-781出版社
SPRINGER
DOI: 10.1007/s10529-013-1407-z
关键词
Complex nitrogen source; Cupriavidus necator; Poly(3-hydroxybutyrate); Waste frying oil; Whey hydrolysate
资金
- Centre for Materials Research at FCH BUT'' from European Regional Development Fund (ERFD) [CZ.1.05/2.1.00/01.0012]
- BUT [CZ.1.07./2.3.00/30.0039]
Whole whey hydrolyzed by Alcalase (WWH) was tested as a complex nitrogen source for the production of poly(3-hydroxybutyrate) (PHB) from waste frying oils by Cupriavidus necator H16. Addition of WWH (10 % (v/v) of cultivation media) supported the growth and PHB accumulation; PHB yields in Erlenmeyer flasks were more than 3.5-fold higher than in control cultivations. The positive influence of WWH on PHB production was confirmed in experiments performed in laboratory fermentor. C. necator cultivated with WWH produced 28.1 g PHB l(-1) resulting in a very high product yield coefficient of 0.94 g PHB per g oil. Since PHB yields were similar to 40 % higher than in the control cultivation, WWH can be considered as an excellent inexpensive nitrogen source for PHB production by C. necator.
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