期刊
BIOTECHNOLOGY LETTERS
卷 35, 期 9, 页码 1425-1432出版社
SPRINGER
DOI: 10.1007/s10529-013-1219-1
关键词
Biosynthesis; Pigment; Monascus ruber; Regulator; trpC promoter
资金
- Natural Science Foundation of China [31171649/C200101]
Pigments produced by Monascus are traditional food colorants and are widely used as dietary supplements. Since genes involving in pigment biosynthesis have not been reported, we describe the identification of a putative pigment-regulatory gene (pigR) obtained by molecular analysis of an albino strain of Monascus ruber M7. In the pigR-deleted strain (Delta pigR), neither the pigments nor pigR expression were detected by HPLC or reverse-transcription PCR, respectively, whereas the introduction of the pigR, together with a constitutive trpC promoter into Delta pigR, caused it to produce 5.4 U of red pigments/g dry mycelia, about 12-fold higher than Monascus ruber M7 (0.46 U/g dry mycelia). Thus pigR up-regulates pigment production in Monascus ruber M7.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据