期刊
BIOTECHNOLOGY LETTERS
卷 35, 期 8, 页码 1191-1197出版社
SPRINGER
DOI: 10.1007/s10529-013-1198-2
关键词
Baculovirus expression system (BES); Ferroportin; Hepcidin; Iron metabolism
资金
- Golestan University of Medical Sciences
- Isfahan University of Medical Sciences
Hepcidin is a peptide hormone that plays an important role in iron metabolism. We have produced a recombinant mouse hepcidin-1 by using baculovirus expression system. Its expression yield was 25 mu g/ml when cell culture media were supplemented with a protease inhibitor cocktail. The recombinant mouse hepcidin-1 and synthetic human hepcidin-25 had similar effects on reducing ferroportin expression in J774A cell line and in peritoneal macrophages. However, synthetic human hepcidin-25 was more efficient than recombinant mouse hepcidin-1 in reducing iron concentration in blood circulation (p < 0.01).
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