期刊
BIOTECHNOLOGY LETTERS
卷 35, 期 12, 页码 2201-2212出版社
SPRINGER
DOI: 10.1007/s10529-013-1313-4
关键词
Induced pluripotent stem cells; Low-intensity pulsed ultrasound; Nerve repair; Neural differentiation; Peripheral nerve repair
资金
- National Natural Science Foundation of China [11172338]
- Program for New Century Excellent Talents in University [NCET-10-0879]
- Fundamental Research Funds for the Central Universities [CDJZR 12238801, CQDXWL-2012-Z001]
- Chongqing University
Low-intensity pulsed ultrasound (LIPUS) acting on induced pluripotent stem cells-derived neural crest stem cells (iPSCs-NCSCs) is considered a promising therapy to improve the efficacy of injured peripheral nerve regeneration. Effects of LIPUS on cell viability, proliferation and neural differentiation of iPSCs-NCSCs were examined respectively in this study. LIPUS at 500 mW cm(-2) enhanced the viability and proliferation of iPSCs-NCSCs after 2 days and, after 4 days, up-regulated gene and protein expressions of NF-M, Tuj1, S100 beta and GFAP in iPSCs-NCSCs whereas after 7 days expression of only NF-M, S100 beta and GFAP were up-regulated. LIPUS treatment at an appropriate intensity can, therefore, be an efficient and cost-effective method to enhance cell viability, proliferation and neural differentiation of iPSCs-NCSCs in vitro for peripheral nerve tissue engineering.
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