期刊
BIOTECHNOLOGY LETTERS
卷 34, 期 10, 页码 1857-1862出版社
SPRINGER
DOI: 10.1007/s10529-012-0972-x
关键词
Biocontrol; Major facilitator superfamily; Self-protection; Trichoderma harzianum; Trichodermin
资金
- Zhejiang Provincial Natural Foundation of China [Y307128]
- Key Science and Technology Project of Zhejiang Province [2008C02007-2]
A novel major facilitator superfamily (MFS) transporter gene, Thmfs1, was isolated from Trichoderma harzianum (T. harzianum). A Thmfs1 over-expressing mutant displayed enhanced antifungal activity and fungicide tolerance, while the Thmfs1 disruption mutant showed the opposite trend. Trichodermin production in Thmfs1 disruption group (185 mg l(-1)) was decreased by less than 17 % compared to the parental strain, suggesting that Thmfs1 is not mainly responsible for trichodermin secretion. Real-time PCR showed that Thmfs1 transcript level could be induced by a certain range of trichodermin concentrations, while expression of Tri5, encoding a trichodiene synthase, was strongly inhibited under these conditions. To our knowledge, Thmfs1 is the first MFS transporter gene identified in T. harzianum.
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