期刊
BIOTECHNOLOGY LETTERS
卷 34, 期 11, 页码 2119-2124出版社
SPRINGER
DOI: 10.1007/s10529-012-1019-z
关键词
Carbon skeleton; C-13-NMR; Haloroquinone; Halorosellinia sp.
资金
- National High Technology Research and Development Program of China [2011AA090702, 2012AA092103, 2012AA092105]
- Shanghai Leading Academic Discipline Project [B505]
Purpose of work: The biosynthetic pathway of a new antitumor compound, haloroquinone, is elucidated to facilitate metabolic regulation for product accumulation and modification to produce new bioactive structural analogues of the compound. The biosynthetic origin of a novel promising protein kinase B inhibitor and anti-tumor compound, haloroquinone, from a marine-derived fungus, Halorosellinia sp. was clarified. The origin of carbon skeleton of haloroquinone was elucidated by feeding experiments with [2-C-13] malonate and [1,2,3-C-13(3)] malonate followed by C-13-NMR analysis of the isolated compounds: 15 carbon atoms were derived from malonate, of which eight were from the methylene group and seven from the carboxyl group. The remaining one is probably obtained by O-methylation. Haloroquinone is thus synthesized via a polyketide pathway using malonyl-CoA as both the starter unit and the extender unit.
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