期刊
BIOTECHNOLOGY LETTERS
卷 35, 期 3, 页码 309-314出版社
SPRINGER
DOI: 10.1007/s10529-012-1086-1
关键词
Cell-free synthesis; Encephalomyocarditis virus; RNA virus; Transmission electron microscopy; Virus particles; Virus synthesis
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) [20112006]
- MEXT [22560774]
- Grants-in-Aid for Scientific Research [22560774] Funding Source: KAKEN
Virus particles are promising vehicles and templates for vaccination, drug delivery and material sciences. Although infectious picornaviruses can be synthesized from genomic or synthetic RNA by cell-free protein expression systems derived from mammalian cell extract, there has been no direct evidence that authentic viral particles are indeed synthesized in the absence of living cells. We purified encephalomyocarditis virus (EMCV) synthesized by a HeLa cell extract-derived, cell-free protein expression system, and visualized the viral particles by transmission electron-microscopy. The in vitro-synthesized EMCV particles were indistinguishable from the in vivo-synthesized particles. Our results validate the use of the cell-free technique for the synthesis of EMCV particles.
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