期刊
BIOTECHNOLOGY LETTERS
卷 35, 期 3, 页码 463-469出版社
SPRINGER
DOI: 10.1007/s10529-012-1083-4
关键词
Adherent cells; Mesenchymal stem cells; Non-adherent cells; Pour-off cultures; Static cultures; Suspension cultures
资金
- UK Engineering and Physical Sciences Research Council (EPSRC) [EP/E053556/01, EP/E036252/1]
- University of Sheffield
- EPSRC [EP/E053556/1, EP/E036252/1] Funding Source: UKRI
- Engineering and Physical Sciences Research Council [EP/E036252/1, EP/E053556/1] Funding Source: researchfish
Bone marrow-derived mesenchymal stem cells (BMD-MSCs) are of great interest for tissue engineering, but require expansion before they can be used for therapeutic applications. We compared three different culture techniques for their potential for large scale expansion of rat BMD-MSCs, i.e. monolayer cultures, stirred suspension cultures and pour-off cultures, and found that pour-off cultures supported the biggest expansion in BMD-MSCs as measured by the fibroblastic-colony forming unit assay (CFU-f). BMD-MSCs expanded in stirred suspension cultures stopped proliferating altogether and, although monolayer cultures allowed for expansion of BMD-MSCs, they favoured a differentiated phenotype over uncommitted MSCs. Only BMD-MSCs expanded in pour-off cultures were able to differentiate into both osteoblastic and adipocytic lineages and maintain CFU-f numbers. These data suggest that pour-off cultures are a viable method of BMD-MSC expansion.
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