期刊
BIOTECHNOLOGY LETTERS
卷 34, 期 3, 页码 527-531出版社
SPRINGER
DOI: 10.1007/s10529-011-0794-2
关键词
Escherichia coli; Metabolic engineering; RyhB; sRNA
资金
- National Natural Science Foundation of China [31070092]
- National Basic Research Program of China [2011CB707405]
- Key Scientific and Technological Program of Shandong province [2010GSF10202]
Small RNA (RyhB) was overexpressed artificially using an arabinose-inducible system in Escherichia coli and resulted in more succinate (7-fold) accumulation, which suggested that RyhB had a strong effect on sdhCDAB genes. Acetate was also increased indicating that RyhB had a comprehensive influence on glucose central metabolism. RyhB might therefore be useful for metabolic engineering of E. coli.
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