期刊
BIOTECHNOLOGY LETTERS
卷 33, 期 9, 页码 1737-1744出版社
SPRINGER
DOI: 10.1007/s10529-011-0640-6
关键词
Functional screening; Neuronal differentiation; Random library; SiRNA library
资金
- Natural Science Foundation of China [30570388]
- Major State Basic Research Development Program of China (973 Program) [2007CB946901]
- ShaanXi Scientific Research Grant [2007C219]
Directed neuronal differentiation is crucial for development of cell therapy and investigation of neurogenesis. However, limited differentiation-inducing agents are available and most of current differentiation regimens are complicated. Here we carried out a combinatorial high-throughput screen with a random siRNA library on murine P19 cell differentiation toward neuronal lineage. Two siRNAs screened from the library were able to direct neuronal differentiation, determined by nestin, neurofilament-M and MAP-2 up-regulation. This is the first trial of a screening procedure for neuronal differentiation-directing agents arising from a random siRNA library and demonstrates that a random siRNA library can be considered as a new resource in efforts to seek new agents for directed differentiation. As the random siRNA library has a broad coverage for the entire genome, screening using random siRNA library can be expected to greatly augment the repertoire of siRNAs for directed differentiation.
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