期刊
BIOTECHNOLOGY LETTERS
卷 33, 期 6, 页码 1215-1219出版社
SPRINGER
DOI: 10.1007/s10529-011-0560-5
关键词
Glycosidases; Ionic liquid; Thermotoga maritima; Thermus thermophilus
资金
- ANR [ANR-07-CP2D-Glycenli]
- France/Algeria grant
The activity and stability of a beta-glycosidase (Thermus thermophilus) and two alpha-galactosidases (Thermotoga maritima and Bacillus stearothermophilus) were studied in different hydrophilic ionic liquid (IL)/water ratios. For the ILs used, the glycosidases showed the best stability and activity in 1,3-dimethylimidazolium methyl sulfate [MMIM][MeSO(4)] and 1,2,3-trimethylimidazolium methyl sulfate [TMIM] [MeSO(4)]. A close correlation was observed between the thermostability of the enzymes and their stability in IL media. At high IL concentration (80%), a time-dependent irreversible denaturing effect was observed on glycosidases while, at lower concentration (<30%), a reversible inactivation affecting mainly the k(cat) was obtained. The results demonstrate that highly thermostable glycosidases are more suitable for biocatalytic reactions in water-miscible ILs.
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