期刊
BIOTECHNOLOGY LETTERS
卷 33, 期 8, 页码 1675-1681出版社
SPRINGER
DOI: 10.1007/s10529-011-0604-x
关键词
Single cell analysis; Oxygen measurement; Ultra-micro electrode; Amperometry; Electrochemical impedance spectroscopy
资金
- Stanford University
- Ministry of Environment
The oxygen evolution of single cells was investigated using a nano-probe with an ultra-micro electrode (UME) in a submicron sized system in combination with a micro-fluidic system. A single cell was immobilized in the micro-fluidic system and a nano-probe was inserted into the cytosolic space of the single cell. Then, the UME was used for an in vivo amperometric experiment at a fixed potential and electrochemical impedance spectroscopy to detect oxygen evolution of the single cell under various light intensities.
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