期刊
BIOTECHNOLOGY LETTERS
卷 33, 期 8, 页码 1615-1619出版社
SPRINGER
DOI: 10.1007/s10529-011-0596-6
关键词
Chelex-100; Chlorella; Colony polymerase chain reaction; Genetic screening; Microalgae
资金
- Chinese Natural Science Foundation For Distinguished Group [50621063]
- Central South University [2010bsxt05]
A colony PCR technique was applied for both genomic and chloroplast DNA in the green microalgae Chlorella. Of five different lysis buffers, Chelex-100 was superior for DNA extraction, PCR and DNA storage. It also was insensitive to variations in cell density. The conditions established for an improved PCR formulation are applicable for screening of genetically-engineered transformants as well as bioprospecting of natural microalgal isolates. Besides multiple Chlorella species, we also demonstrate the efficacy of Chelex-100 for colony PCR with a number of other microalgal strains, including Chlamydomonas reinhardtii, Dunaliella salina, Nannochloropsis sp., Coccomyxa sp., and Thalassiosira pseudonana.
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