期刊
BIOTECHNOLOGY LETTERS
卷 32, 期 12, 页码 1961-1968出版社
SPRINGER
DOI: 10.1007/s10529-010-0362-1
关键词
Anti-sense; Bamboo EMF2; Rapid amplification of cDNA ends; Real-time PCR; Rescue
资金
- Chinese Academy of Sciences [KSCX2-YW-N-029]
- National Basic Research Program of China (973 Program) [2007CB411600]
- National Natural Science Foundation of China [30770154]
A novel EMBRYONGIC FLOWER2 gene, DlEMF2, was isolated from bamboo, Dendrocalamus latiflorus. It is 2518 bp in length encoding a protein of 629 amino acids. It was expressed in all organs and at a higher level in shoot tissue than in inflorescences. Transgenic Arabidopsis plants expressing antisense DlEMF2 displayed a spectrum of early-flowering phenotypes. Quantitative real-time PCR experiments indicated that the antisense transgene DlEMF2 reduced endogenous AtEMF2 transcript levels leading to the flowering time reduction. Besides, transforming the bamboo DlEMF2 gene into Arabidopsis emf2-1 mutant could rescue phenotype in vegetative and reproductive developmental stages, which reveals that EMF2 has functional conservation between monocots and eudicots.
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