期刊
BIOTECHNOLOGY LETTERS
卷 32, 期 12, 页码 1925-1932出版社
SPRINGER
DOI: 10.1007/s10529-010-0376-8
关键词
Cupriavidus necator; Polyhydroxyalkanoates; Propanol; Stress conditions; Waste edible oils
资金
- Czech Ministry of Education [MSM 0021630501, CZ.1.05/2.1.00/01.0012]
Waste rapeseed oil is a useful substrate for polyhydroxyalkanoates (PHA) production employing Cupriavidus necator H16. In fed-batch mode, we obtained biomass and PHA yields of 138 and 105 g l(-1), respectively. Yield coefficient and volumetric productivity were 0.83 g PHA per g oil and 1.46 g l(-1) h(-1), respectively. Propanol at 1% (v/v) enhanced both PHA and biomass formation significantly and, furthermore, resulted in incorporation of 3-hydroxyvalerate units into PHA structure. Thus, propanol can be used as an effective precursor of 3-hydroxyvalarete for production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer. During the fed-batch cultivation, propanol concentration was maintained at 1% which resulted in 8% content of 3-hydroxyvalerate in copolymer.
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