期刊
BIOTECHNOLOGY LETTERS
卷 32, 期 6, 页码 867-875出版社
SPRINGER
DOI: 10.1007/s10529-010-0236-6
关键词
Dengue virus; Envelope protein; Magnifection; Nicotiana benthamiana; Transient expression
资金
- University of Buenos Aires
- CONICET (National Council of Scientific and Technical Research)
Dengue virus envelope glycoprotein (E-protein) is the main protein associated with immunity induction. To produce a candidate for subunit vaccines and to provide an antigen for diagnostic kits, it was expressed in a novel plant system using deconstructed viral modules. A truncated version of the E-protein was designed to be expressed alone and co-expressed with Dengue virus structural proteins. As well, the critical domain III of E-protein was fused to hepatitis B core antigen (HBcore). The recombinant proteins were produced in Nicotiana benthamiana plants and were reactive with the anti-E antibody. The fusion was reactive with both anti-E and anti-HBcore antibodies.
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