期刊
BIOTECHNOLOGY LETTERS
卷 32, 期 12, 页码 1789-1796出版社
SPRINGER
DOI: 10.1007/s10529-010-0359-9
关键词
Adipocytes; Bioassay; Bisphenol A; Recombinant peroxisome proliferator response element-driven luciferase; Phthalates
资金
- National Science Council [NSC97-2314-B-400-003-MY3]
- National Health Research Institutes in Taiwan [EO-097-PP-07, EO-98-PP-05]
A recombinant Huh7-PPRE-Luc cell line for analyzing the peroxisome proliferator response element (PPRE)-driven luciferase activity was established. The cells exhibited a good dose-response induction in PPRE-driven luciferase activity by three subtypes of peroxisome proliferator-activated receptor (PPAR) agonists as well as by a retinoid X receptor agonist, 9-cis-retinoic acid. Among five environmental chemicals tested, benzyl butyl phthalate and bisphenol induced PPRE-driven luciferase activation in Huh7-PPRE-Luc cells and caused adipogenic differentiation of 3T3-L1 cells. This recombinant Huh7-PPRE-Luc cell line would be useful for screening potential environmental obesogens with PPAR activity.
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