期刊
BIOTECHNOLOGY LETTERS
卷 33, 期 3, 页码 443-456出版社
SPRINGER
DOI: 10.1007/s10529-010-0469-4
关键词
Aggregates; Enzymes; Human therapeutics; Interfaces; Proteins; Shear
The effects of shear on proteins in solution are described and discussed. Research on this topic covers many decades, beginning with investigations of possible denaturation of enzymes during processing, whilst more recent concerns are how the quality of therapeutic proteins might be affected by shear or shear related effects. The paradigm that emerges from most studies is that shear in the fluid mechanical sense is unlikely by itself to damage most proteins and that interfacial phenomena are critically important. In particular, moving gas-liquid interfaces can be very deleterious. Aggregation of therapeutic proteins on nanoparticles shed from solid surfaces is a recent concern because of potential consequences on patient safety. It is clear that labeling such damage as shear is a mistake as this inhibits clear investigations of, and thinking about, the true causes of damage to proteins in solution during processing.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据