期刊
BIOTECHNOLOGY LETTERS
卷 32, 期 9, 页码 1223-1229出版社
SPRINGER
DOI: 10.1007/s10529-010-0295-8
关键词
Baculovirus; Codon optimization; Foot-and-mouth disease virus; Virus-like particles
资金
- Chinese 863'' National Program for High Technology Research and Development [2006AA10A204]
A recombinant baculovirus was constructed to simultaneously express codon-optimized virus-like particles (VLP), A VP1-2A-VP3 and VP0 of serotype O foot-and-mouth disease virus (FMDV), from individual promoters. The target proteins were expressed in insect cells at high level, as shown by indirect sandwich ELISA; and the expressed VP1-2A-VP3 could autocatalytically be cleaved into the individual proteins, VP1-2A and VP3, as shown by Western-blot analyses. In addition, in the insect cells, the structural proteins, VP0, VP3 and VP1-2A, self-assembled into virus-like particles resembling the authentic FMDV particles. This information should prove useful for the development of more efficient VLP assembly using shorter genes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据