期刊
BIOTECHNOLOGY LETTERS
卷 31, 期 8, 页码 1295-1301出版社
SPRINGER
DOI: 10.1007/s10529-009-9992-6
关键词
Aspergillus ficuum; Phytase; Root-specific expression; Secretory protein; Transgenic soybean
资金
- Natural Science Foundation of Hebei Province [C2006000559]
- Science and Technology Department of Hebei Province [06547005B-3]
Phytases release inorganic phosphates from phytate in soil. A gene encoding phytase (AfPhyA) was isolated from Aspergillus ficuum and its ability to degrade phytase and release phosphate was demonstrated in Saccharomyces cerevisiae. A promoter from the Arabidopsis Pky10 gene and the carrot extensin signal peptide were used to drive the root-specific and secretory expression of the AfPhyA gene in soybean plants. The phytase activity and inorganic phosphate levels in transgenic soybean root secretions were 4.7 U/mg protein and 439 mu M, respectively, compared to 0.8 U/mg protein and 120 mu M, respectively, in control soybeans. Our results demonstrated the potential usefulness of the root-specific promoter for the exudation of recombinant phytases and offered a new perspective on the mobilization of phytate in soil to inorganic phosphates for plant uptake.
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