期刊
BIOTECHNOLOGY LETTERS
卷 32, 期 4, 页码 547-555出版社
SPRINGER
DOI: 10.1007/s10529-009-0179-y
关键词
Agrobacterium; Cotton; Pistil; Transformation
资金
- National High Technology Research and Development Program of China [2006AA100105]
- Science & Technology Pillar Program of Jiangsu Province [BE2008310]
- Programme of Introducing Talents of Discipline to Universities [B08025]
Transgenic cotton plants were developed by pistil drip inoculation in a solution containing Agrobacterium carrying a gene for resistance to the herbicide Basta (bar), 10% (w/v) sucrose, 0.05% (v/v) Silwet L-77 and 40 mg acetosyringone l(-1). Pistil drip during 17:00-19:00 on the first day of flowering resulted in 0.07-0.17% Basta-resistant plants/number of viable seeds generated, and stigma excision prior to pistil drip during this time period gave rise to a transformation efficiency of 0.46-0.93%, in contrast with 0.04-0.06% generated from pistil drip during 9:00-11:00 on the second day of flowering. PCR and Southern blot analysis confirmed the integration of the bar gene into the cotton genome, and a T1 and T2 generation herbicide resistance test consistently revealed expression and stable heritability of the bar gene in the two generations.
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