期刊
BIOTECHNOLOGY LETTERS
卷 31, 期 7, 页码 979-984出版社
SPRINGER
DOI: 10.1007/s10529-009-9963-y
关键词
DPP-IV inhibitor; High throughput screening (HTS); Diatetes; Recombinant human dipeptidyl peptidase IV (rhDPP-IV)
资金
- Hi-Tech Research and Development (863) Program of China [2002AA27343B]
Dipeptidyl peptidase (DPP) IV inhibitors provide a new strategy for the treatment of type 2 diabetes. Human DPP-IV gene was cloned from differentiated Caco-2 cells and expressed in Pichia pastoris. The recombinant enzyme was used in a new system for screening of DPP-IV inhibitors. By high throughput screening, a novel compound (W5188) was identified from 75,000 compounds with an IC50 of 6.5 mu M. This method is highly reproducible and reliable for discovery of DPP-IV inhibitors as shown by Z' value of 0.73 and S/N ratio of 6.89.
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