期刊
BIOTECHNOLOGY LETTERS
卷 32, 期 2, 页码 181-187出版社
SPRINGER
DOI: 10.1007/s10529-009-0137-8
关键词
IL-6; Low O-2 tension; MMP-1; Proliferation; Tenocyte culture
资金
- [2005CB522703]
- [2006A A02A127]
- [09ZR1416700]
- [2008XJ026]
Tenocytes reside in relatively avascular tissue and are difficult to expand due to phenotype drift and functional loss. Thus low O-2 tension culture was employed to enhance the expansion capability. The results demonstrated that low O-2 tension (2% O-2) culture could significantly enhance the expansion of newborn pig tenocytes with 275-473% greater cell yield per cell passage that that of regular O-2 cultured (21% O-2) cells. Importantly, low O-2 culture did not change the gene expression of functional molecule such as collagens I and III, decorin, prolyl 4-hydroxylase (P4H), lysyl oxidase (LOX), TIMP-1 and TIMP-2, but could significantly down regulate the gene expression of MMP-1 and IL-6. In conclusion, low O-2 tension culture can significantly enhance the expansion capacity of tenocytes without affecting their phenotype and functions.
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