期刊
BIOTECHNOLOGY LETTERS
卷 31, 期 12, 页码 1923-1927出版社
SPRINGER
DOI: 10.1007/s10529-009-0094-2
关键词
Fusion protein; Hirudin; Staphylokinase; Thrombosis
Staphylokinase (SAK) is an effective thrombolysis agent for therapy of myocardial infarction. We have constructed a fusion SAK variant (SAK-HV) with a thrombin-binding domain composed of 12 amino acids from hirudin and expressed it in Escherichia coli and purified the resultant protein. SAK-HV maintained fibrinolytic activity similar to SAK and had anticoagulant activity attributable to its hirudin segment. Measurement of thrombin-binding activity in vitro demonstrated that SAK-HV possessed binding activity with thrombin while SAK did not. SAK-HV might thus be a more potent thrombolytic agent with anticoagulation property than SAK.
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