期刊
BIOTECHNOLOGY LETTERS
卷 32, 期 2, 页码 315-319出版社
SPRINGER
DOI: 10.1007/s10529-009-0143-x
关键词
alpha-Amyrin; Asiaticoside; Centella asiatica; Madecassoside
资金
- DGPA
- CONACYT
- Spanish Ministerio de Ciencia e Innovacion [BIO2008-01210]
- Generalitat de Catalunya [2009SGR1217]
Plant cell cultures of Centella asiatica produce small quantities of centellosides: madecassosid > asiaticosid > madecassic acid > asiatic acid. To obtain a more efficient production system of these bioactive triterpenoid compounds, we developed a process where the substrate, alpha-amyrin, was converted into centellosides by cell suspensions of C. asiatica. When alpha-amyrin in acetone was added at 0.01 mg/ml(-1) to the culture medium, together with the permeabilizing agent DMSO, after 7 days nearly 50% had penetrated the plant cells, of which almost 84% was transformed into centellosides. The system therefore efficiently converts alpha-amyrin into centellosides, thus opening a new possibility for the production of these compounds.
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