期刊
BIOTECHNOLOGY LETTERS
卷 32, 期 3, 页码 341-350出版社
SPRINGER
DOI: 10.1007/s10529-009-0173-4
关键词
Biocatalysis; Carrier-free immobilization; Cross-linked enzyme aggregate; Pull-down domain; Self-assembling systems
资金
- project for scientific and technological co-operation between Austria and Slovakia [SK-AT-0024-08]
Methods for the preparation of carrier-free insoluble enzymes are reviewed. The technology of cross-linked enzyme aggregates has now been applied to a range of synthetically useful activities. Fusion proteins are also gaining momentum because they allow a relatively selective aggregation or even a specific self-assembly of the desired enzyme activity into insoluble particles in the absence of potentially denaturing chemicals required for precipitation and cross-linking. Recycling of insoluble protein particles for multiple rounds of batchwise reaction has been demonstrated in selected biotransformations. However, for application in a fully continuous biocatalytic process, low resistance to mechanical stress and high compressibility are issues for consideration on carrier-free enzyme particles.
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