期刊
BIOTECHNOLOGY LETTERS
卷 30, 期 9, 页码 1559-1564出版社
SPRINGER
DOI: 10.1007/s10529-008-9723-4
关键词
ammonium ions; docosahexaenoic acid; lipids; pH-auxostat; polyunsaturated fatty acids; Schizochytrium; single cell oils
资金
- Tomas de Zarate Grant (Cabildo de Gran Canaria)
- EU [Q5RS-2000-30271(PUFAfeed)]
Thraustochytrids, in particular Schizochytrium spp., are used for the production of the valuable polyunsaturated fatty acid, docosahexaenoic acid (DHA; 22:6 n-3). Growth of Schizochytrium sp. G13/2S in a defined medium was initially made in shake-flask cultures to determine the optimum concentrations of glucose (100-200 g l(-1)) and ammonia (similar to 300 mg l(-1)) that could be used by this microorganism. In subsequent fermenter cultures, a pH-auxostat method was used to maintain NH3 from 200-300 mg l(-1). During the first 49 h of fermentation, 150 g glucose l(-1) produced 63 g cell dry wt l(-1). Although growth was not limited by the supply of nitrogen, total fatty acids were at 25% cell dry wt which is more than half the final lipid content of commercially-grown Schizochytrium biomass which uses N-limited medium in the final stages for maximum lipid accumulation. This strategy is therefore useful for the cultivation of Schizochytrium to a high cell density up to the point when lipid accumulation can be triggered by N exhaustion.
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