期刊
BIOTECHNOLOGY LETTERS
卷 30, 期 10, 页码 1833-1838出版社
SPRINGER
DOI: 10.1007/s10529-008-9757-7
关键词
high-affinity phosphate transporter; Oryza sativa; phosphate acquisition; ricetillers
资金
- Korea Research Foundation of the Korean Government [KRF-2003-015-C00640]
- National Research Foundation of Korea [2003-015-C00640] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Most high-affinity phosphate transporter genes (OsPTs) in rice were highly induced in roots when phosphate was depleted. OsPT1, however, was highly expressed in primary roots and leaves regardless of external phosphate concentrations. This finding was confirmed histochemically using transgenic rice plants that express the GUS reporter gene under the control of the OsPT1 promoter, which exhibited high GUS activity even in the phosphate sufficient condition. Furthermore, transgenic rice plants overexpressing the OsPT1 gene accumulated almost twice as much phosphate in the shoots as did wild-type plants. As a result, transgenic plants had more tillers than did wild-type plants, which is a typical physiological indicator for phosphate status in rice.
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