期刊
BIOTECHNOLOGY LETTERS
卷 30, 期 11, 页码 1901-1908出版社
SPRINGER
DOI: 10.1007/s10529-008-9776-4
关键词
A-form helix; melting temperature; 2 '-ribose modification; RNA interference; siRNA; stability
资金
- Korea Food and Drug Administration [07132KFDA689]
Small interfering RNAs (siRNAs) which mediate sequence-specific gene silencing through RNA interference have been harnessed for therapeutic applications. Here, we found combinations of modest 2'-ribose siRNA modifications that permit effective gene silencing and improve siRNA stability in human serum. Introduction of as few as two different or identical 2'-ribose modifications at a specific position of either strand, which has been previously suggested to suppress unintended off-target effects, enhances the thermal and thermodynamic stability of siRNA duplexes, and does not alter the overall A-form helical geometry that is required for silencing activity. Our results provide a useful template for the design for therapeutic siRNAs.
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