期刊
BIOTECHNOLOGY LETTERS
卷 30, 期 12, 页码 2063-2069出版社
SPRINGER
DOI: 10.1007/s10529-008-9818-y
关键词
Expression; Recombinant human factor IX; Transgenic mice
资金
- Empresa Brasileira de Pesquisa Agropecuaria (Embrapa)
- Hospital de Apoio de Brasilia
- Universidade Federal de Sao Paulo
- CNPq
- International Hemophilia Training Center-World Federation of Hemophilia
Human factor IX is synthesized in the liver and secreted in the blood, where it participates in a group of reactions involving coagulation factors and proteins that permit sanguinary coagulation. In this work two lines of transgenic mice were developed to express the FIX gene in the mammalian glands under control of milk beta-casein promoter. The founding females secreted the FIX in their milk (3% total soluble protein). The stable integration of transgene was confirmed by southern blot analysis. The presence of the FIX recombinant protein in the milk of transgenic females was confirmed by western blot and the clotting activity was revealed in blood-clotting assays. The coagulation activity in human blood treated with recombinant FIX increased while the time of coagulation decreased. Our results confirm the production of a large amount of recombinant biologically active FIX in the mammary gland of transgenic mice.
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