期刊
BIOTECHNOLOGY JOURNAL
卷 8, 期 1, 页码 32-+出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201100486
关键词
Biocatalysis; Biodegradation; Biosensors; Cell imaging; Protein analysis
资金
- Grant Agency of the Czech Republic [203/08/0114, P503/12/0572, P207/12/0775]
- Grant Agency of the Czech Academy of Sciences [IAA401630901]
- European Regional Development Fund [CZ.1.05/2.1.00/01.0001]
- Brno City Murlicipality
Haloalkane dehalogenases (EC 3.8.1.5, HLDs) are a/beta-hydrolases which act to cleave carbon-halogen bonds. Due to their unique catalytic mechanism, broad substrate specificity and high robustness, the members of this enzyme family have been employed in several practical applications: (i) biocatalytic preparation of optically pure building-blocks for organic synthesis; (ii) recycling of by-products from chemical processes; (iii) bioremediation of toxic environmental pollutants; (iv) decontamination of warfare agents; (v) biosensing of environmental pollutants; and (vi) protein tagging for cell imaging and protein analysis. This review discusses the application of HLDs in the context of the biochemical properties of individual enzymes. Further extension of HLD uses within the field of biotechnology will require currently limiting factors such as low expression, product inhibition, insufficient enzyme selectivity, low affinity and catalytic efficiency towards selected substrates, and instability in the presence of organic co-solvents to be overcome. We propose that strategies based on protein engineering and isolation of novel HLDs from extremophilic microorganisms may offer solutions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据