期刊
BIOTECHNOLOGY JOURNAL
卷 5, 期 9, 页码 978-985出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/biot.201000132
关键词
High-throughput cloning; Linear vector; Membrane protein; Mistic; Mono Q (TM)
资金
- European grant [LSMH-CT-EUR-INTAFAR 2004-512138, ANR-FORM-080124-01-01]
In this report we describe a rapid, simple, and efficient method for large-scale purification of linear plasmid DNA to answer demand from high-throughput gene cloning. The process is based on the separation of the linear vector from small DNA fragments by anion exchange chromatography. Gene cloning experiments by restriction/ligation or the In-Fusion (TM) technique confirmed the high quality of the linearized vector as 100% of the genes were successfully cloned.
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