期刊
BIOTECHNOLOGY FOR BIOFUELS
卷 5, 期 -, 页码 -出版社
BMC
DOI: 10.1186/1754-6834-5-44
关键词
Clostridium acetobutylicum; Butanol tolerance; Isopropanol-butanol-ethanol (IBE) fermentation; Secondary alcohol dehydrogenase; Acetone-butanol-ethanol (ABE) fermentation
资金
- National High Technology Research and Development Program of China (863 program) [2011AA02A208]
- National Basic Research Program of China (973 program) [2011CBA00807]
- Chinese Academy of Sciences [KSCX2-EW-Q-14]
Background: Previously we have developed a butanol tolerant mutant of Clostridium acetobutylicum Rh8, from the wild type strain DSM 1731. Strain Rh8 can tolerate up to 19 g/L butanol, with solvent titer improved accordingly, thus exhibiting industrial application potential. To test if strain Rh8 can be used for production of high level mixed alcohols, a single secondary alcohol dehydrogenase from Clostridium beijerinckii NRRL B593 was overexpressed in strain Rh8 under the control of thl promoter. Results: The heterogenous gene sADH was functionally expressed in C. acetobutylicum Rh8. This simple, one-step engineering approach switched the traditional ABE (acetone-butanol-ethanol) fermentation to IBE (isopropanol-butanol-ethanol) fermentation. The total alcohol titer reached 23.88 g/l (7.6 g/l isopropanol, 15 g/l butanol, and 1.28 g/l ethanol) with a yield to glucose of 31.42%. The acid (butyrate and acetate) assimilation rate in isopropanol producing strain Rh8(psADH) was increased. Conclusions: The improved butanol tolerance and the enhanced solvent biosynthesis machinery in strain Rh8 is beneficial for production of high concentration of mixed alcohols. Strain Rh8 can thus be considered as a good host for further engineering of solvent/alcohol production.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据