期刊
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
卷 19, 期 3, 页码 534-544出版社
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-014-0081-x
关键词
bacterial cellulose; chitosan; release; keratinocytes; fibroblasts
资金
- Ratchadaphiseksomphot Endowment Fund of Chulalongkorn University [RES560530044-AM]
- Ratchadaphiseksomphot Endowment Fund
- Commission on Higher Education (CHE)
- Ratchadapiseksomphot Endowment Fund of Chulalongkorn University [RES560530156-HR]
- Thailand National Science and Technology Development Agency (NSTDA)
Bacterial cellulose-chitosan (BC-C) films were developed by immersing purified BC pellicles in 1.5 similar to 2.0% (w/v) acetic acid solutions containing chitosan of varying molecular weights. Effects of different molecular weight of chitosan on physical, biological and antimicrobial properties of the composite films were investigated. The cumulative chitosan absorption capacities with M-w of 141,000, 199,000, and 263,000 were 38.43, 24.65, and 23.89 mg/cm(3) of dry BC film, respectively. The cumulative release profiles of chitosan from the films strongly depended on molecular weight of chitosan and pH of solution. The order of release of chitosan from the BC-C films was dependent on molecular weight as follows: M-w 141,000 > M-w 199,000 > M-w 263,000. All BC-C films showed the antimicrobial abilities against Staphylococcus aureus and Aspergillus niger but had no inhibitory effect on the growth of Escherichia coli. The BC-C films supported for adhesion, spreading and proliferation of both human skin keratinocytes and fibroblasts. The antibacterial activity against S. aureus of the BC-C with the highest Mw chitosan (263,000) was higher than those of the others. On the other hand, the BC-C films with the lowest M-w chitosan (141,000) promoted the growth of human skin cells more than those of the others.
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