期刊
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
卷 19, 期 3, 页码 379-385出版社
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-014-0021-9
关键词
microfluidic device; poly(lactic-co-glycolic acid); endothelial progenitor cells; 3D extracellular matrix; vasculogenesis
资金
- National Research Foundation of Korea (NRF) [NRF-2013R1A1A2A10061422, NRF-2010-0020409]
- NRF [NRF-2012-022481]
- Human Resources Program in Energy Technology of the Korea Institute of Energy Technology Evaluation and Planning [KETEP] [20124010203250]
- NRF-Global Ph. D. Fellowship Program
Vasculogenesis is an important morphogenetic event for vascular tissue engineering and ischemic disease treatment. Stem and progenitor cells can contribute to vasculogenesis via endothelial differentiation and direct participation in blood vessel formation. In this study, we developed an implantable microfluidic device to facilitate formation of three-dimensional (3D) vascular structures by human endothelial progenitor cells (hEPCs). The microfluidic device was made of biodegradable poly(lactic-co-glycolic acid) (PLGA) using a microchannel patterned silicon wafer made by soft lithography. A collagen type I (Col I) hydrogel containing hEPCs filled the microfluidic channels to reconstitute a 3D microenvironment for facilitating vascular structure formation by hEPCs. The device seeded with hEPCs was implanted into the subcutaneous space of athymic mice and retrieved one and four weeks after implantation. Histology and immunohistochemistry revealed that hEPCs formed a 3D capillary network expressing endothelial cell-specific proteins in the channel of the PLGA microfluidic device. This result indicates that a 3D microscale extracellular matrix reconstituted in the microchannel can promote the endothelial differentiation of hEPCs and in turn hEPC-mediated vasculogenesis. The PLGA microfluidic device reported herein may be useful as an implantable tissue-engineering scaffold for vascularized tissue reconstruction and therapeutic angiogenesis.
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