期刊
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
卷 17, 期 6, 页码 1147-1155出版社
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-012-0279-8
关键词
lipase; Burkholderia cepacia G63; resolution; ketoprofen; response surface methodology
资金
- National Natural Science Foundation of China [31070089, 31170078]
- National High Technology Research and Development Program of China [2010AA101501, 2011AA02A204]
- Natural Science Foundation of Hubei Province [2009CDA046]
A lipase from the Burkholderia cepacia strain G63 immobilized on resin was used for the resolution of ketoprofen. To study its catalytic properties in enantioselective esterication, different alcohols and solvents were tested to select the most suitable acyl acceptor and reaction medium. Compared with the low activity of the free lipase, the enzyme activity and E value of the immobilized lipase were significantly enhanced. The enantioselectivity of the immobilized lipase could also be markedly improved by adding a small amount of 18-crown-6. RSM was employed to optimize the reaction parameters. The optimal reaction conditions were: reaction time 22.50 h, additives dosage 0.4322 g (0.33 mmol/mL), and substrate molar ratio 54.11:1. Under optimal conditions, the maximal E value was up to 10.01, which exhibited a better enantioselectivity than some commercial lipases, such as Novozym 435, Lipozyme RM IM and LipozymeTL IM.
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