期刊
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
卷 17, 期 1, 页码 67-75出版社
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-011-0347-5
关键词
microbial lipase; organic solvent-tolerant; Streptomyces; biodiesel production
资金
- Korean Research Foundation (KRF)
- Korean government (MEST) [2010-0029178]
- Technology Development Program for Agriculture and Forestry, Ministry of Agriculture and Forestry, Republic of Korea [307014-5]
- National Research Foundation of Korea [2010-0029178] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
In an effort to identify a microbial lipase that can catalyze transesterification reactions used in biodiesel production, an organic solvent-tolerant lipase was purified from Streptomyces sp. CS268. The molecular weight of the purified lipase was estimated to be 37.5 kDa by SDS-PAGE. The lipase showed highest activity at a temperature of 30A degrees C and pH 8.0 while it was stable in the pH range 4.0 similar to 9.0 and at temperatures a parts per thousand currency sign 50A degrees C. It showed the highest hydrolytic activity towards medium-length acyl chain p-nitrophenyl decanoate with K (m) and V (max) values of 0.59 mM and 319.5 mmol/mg/min, respectively. Also, the lipase showed non-position specificity for triolein hydrolysis. The purified lipase catalyzed transesterification reaction of soybean oil with methanol, suggesting that it can be a potential enzymatic catalyst for biodiesel production.
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