期刊
JOURNAL OF CANCER
卷 9, 期 24, 页码 4677-4683出版社
IVYSPRING INT PUBL
DOI: 10.7150/jca.26461
关键词
long non-coding RNA (lncRNA); AFAP1 antisense RNA 1 (AFAP1-AS1); colon cancer (CC); metastasis; prognosis
类别
资金
- National Natural Science Foundation of China [81572787, 81672683, 81772901, 81702907, 81772928, 81803025]
- Natural Science Foundation of Hunan Province [2016JC2035, 2017SK2015, 2018JJ3704, 2018JJ3815, 2018SK21210, 2018SK21211]
- Fundamental Research Funds for the Central Universities of Central South University [10533201710 23]
- Special Fund of Clinical Medicine of Chinese Medical Association [17020280697]
Long non-coding RNAs (lncRNAs) are dysregulated in various cancers. However, the clinical relevance and functional roles of AFAP1-AS1 in colon cancer (CC) have not been clarified. We analyzed the lncRNA expression patterns in Gene Expression Omnibus (GEO) datasets and the Cancer Genome Atlas (TCGA) RNA-seq datasets, and found that the expression level of AFAP1-AS1 was significantly elevated in CC tissues. High levels of AFAP1-AS1 were associated with poor disease-free survival and overall survival in CC patients. In vitro experiments demonstrated that AFAP1-AS1 knockdown significantly inhibited the cell invasive and migration capability in CC cell line HT-29. AFAP1-AS1 knockdown also increased the expression of E-cadherin and ZO-1 while inhibited the expression of Vimentin, MMP9, ZEB1 and beta-catenin, suggesting that AFAP1-AS1 is involved in the epithelial-mesenchymal transition (EMT) process of CC. Further studies confirmed that AFAP1-AS1 knockdown also affected the actin-cytokeratin signaling pathway. Thus, AFAP1-AS1 might be a potential novel diagnostic marker and therapeutic target for CC.
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