期刊
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
卷 16, 期 1, 页码 107-113出版社
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-010-0161-5
关键词
L-ascorbic acid; beta-cyclodextrin; alpha-cyclodextrin glucanotransferase; 2-O-alpha-D-glucopyranosyl-L-ascorbic acid
资金
- National Science Fund for Distinguished Young Scholars of China [20625619]
- Fundamental Research Funds for the Central Universities [JUSRP30901]
- 973 Project [2007CB714306, 2010CB535014]
The study aimed to produce 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G) via the transglycosylation reaction by alpha-cyclodextrin glucanotransferase (alpha-CGTase) from recombinant Escherichia coli with L-ascorbic acid (AA) and beta-cyclodextrin (beta-CD) as the substrates. Liquid chromatography-tandem mass spectrometry analysis was conducted for AA-2G identification, and the glucoamylase treatment was carried out to produce AA-2G from AA-2-oilgosaccharides. The optimal temperature and pH for the enzymatic AA-2G production were 37 degrees C and 5.5, respectively, and the optimal alpha-CGTase concentration and substrate mass ratio (AA:beta-CD) for AA-2G synthesis were 160 U/mL and 1:1, respectively. At these optimal process conditions, maximal AA-2G production reached 13 g/L. This is the first report regarding the process optimization of enzymatic AA-2G production by alpha-CGTase from recombinant E. coli. The results may be useful for the industrial scale production of AA-2G.
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