期刊
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
卷 15, 期 4, 页码 657-663出版社
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-009-3128-7
关键词
polysialic acid; Escherichia coli; phosphate; ammonia
资金
- National High Technology Research and Development Program of China [2006AA02Z207]
- Research Fund for the Doctoral Program of Higher Education of China [20077434]
Polysialic acid (PSA) is a capsular polysaccharide obtained from aerobic fermentation with Escherichia coli. To enhance PSA production and eliminate the influence of phosphate on the PSA purification process, a lower level of initial phosphate was adopted with pH control. The resulting PSA yield reached 4.1 g/L in fed-batch fermentation with 2.5 g/L K2HPO4 and E. coli strain CCTCC M208088. In addition, an ammonia water (NH4OH) feeding strategy to control the pH at 6.4 was developed resulting in PSA production that reached as high as 5.2 g/L. NMR spectra confirmed the purified biopolymer as a alpha-2,8 linked PSA, identical to the published NMR spectra, with a molecular weight in the range of 16 similar to 50 kDa.
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