期刊
BIOTECHNOLOGY AND BIOPROCESS ENGINEERING
卷 15, 期 6, 页码 984-992出版社
KOREAN SOC BIOTECHNOLOGY & BIOENGINEERING
DOI: 10.1007/s12257-009-3160-7
关键词
alkaline alpha-amylase; Bacillus alcalophilus; magnetic nanoparticles; Plackett-Burman design; response surface; starch hydrolysis
资金
- DBT, New Delhi
- Tezpur University
We report the statistical optimization of the immobilization of alkaline a-amylase [E C 3 2 1 1] from Bacillus alcalophilus onto nano-sized supermagnetic iron-oxide nanoparticles (MNPs) for augmenting the cost effective industrial application of MNP-bound a-amylase Both Plackett-Burman factorial design and response surface methodology (RSM) were employed to screen the influence of different parameters and the central effect of response on the a-amylase-iron oxide MNP binding process The high coefficient of determination (R-2) and analysis of variance (ANOVA) of the quadratic model indicated the competence of the proposed model The size of the MNPs was confirmed by X-ray diffraction and scanning electron microscope analyses in which Fourier transform infrared spectroscopy suggested immobilization of the enzyme on iron-oxide MNPs A significant improvement (similar to 26-fold) in specific activity, thermal and storage stability, and reusability of a-amylase after binding with iron-oxide MNP reinforced the improved biotechnological potential of the cc-amylase iron-oxide MNP bioconjugate compared to free a-amylase These results open new avenues for applying this MNP immobilized enzyme in different industrial sectors, notably in the paper and brewing industries
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