期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 111, 期 1, 页码 152-164出版社
WILEY-BLACKWELL
DOI: 10.1002/bit.24992
关键词
metabolomics; xylose fermentation; inhibitors; cellulosic ethanoln
资金
- National High Technology Research and Development Program (863Program) [2012AA02A701]
- National Natural Science Foundation of China
- National Basic Research Program of China (973 Program) [2013CB733601]
- International Joint Research Project of Tianjin [11ZCGHHZ00500]
- U.S. National Science Foundation [DBI-0619489]
During lignocellulosic ethanol fermentation, yeasts are exposed to various lignocellulose-derived inhibitors, which disrupt the efficiency of hexose and pentose co-fermentation. To understand the metabolic response of fermentation microbes to these inhibitors, a comparative metabolomic investigation was performed on a xylose-fermenting Saccharomyces cerevisiae 424A (LNH-ST) and its parental strain 4124 with and without three typical inhibitors (furfural, acetic acid, and phenol). Three traits were uncovered according to fermentation results. First, the growth of strain 424A (LNH-ST) was more sensitive to inhibitors than strain 4124. Through metabolomic analysis, the variance of trehalose, cadaverine, glutamate and -aminobutyric acid (GABA) suggested that strain 424A (LNH-ST) had a lower capability to buffer redox changes caused by inhibitors. Second, lower ethanol yield in glucose and xylose co-fermentation than glucose fermentation was observed in strain 424A (LNH-ST), which was considered to be correlated with the generation of xylitol, as well as the reduced levels of lysine, glutamate, glycine and isoleucine in strain 424A (LNH-ST). Accumulation of glycerol, galactinol and mannitol was also observed in strain 424A (LNH-ST) during xylose fermentation. Third, xylose utilization of strain 424A (LNH-ST) was more significantly disturbed by inhibitors than glucose utilization. Through the analysis of fermentation and metabolomic results, it was suggested that xylose catabolism and energy supply, rather than xylose uptake, were the limiting steps in xylose utilization in the presence of inhibitors. Biotechnol. Bioeng. 2014;111: 152-164. (c) 2013 Wiley Periodicals, Inc.
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